The γ-Aminobutyric Acid (GABA) Synthesis Gene Regulates the Resistance to Water Core-Induced Hypoxia Stress for Pear Fruits

Watercore is a physiological disorder which often occurs in Rosaceae fruits, and it causes hypoxia stress, promoting fruit decay. γ-aminobutyric acid (GABA) was reported as being involved different abiotic stresses, glutamate decarboxylase (GAD) the key enzyme of GABA synthesis plants. Our previous transcriptome analysis found that PpGAD2 significantly induced watercore fruit; however, mechanism through regulates watercore-induced stress resistance pears unclear. The present study pulp ethanol, malondialdehyde (MDA) H2O2 content inhibited by exogenous GABA. transcript abundance higher than other PpGADs or healthy fruit. Tissue expression showed mature young Moreover, subcellular localization located cytoplasm. Transient overexpression assays suggested had role synthesis. Several CML (calmodulin–like) genes were also increased PpWRKY53 fruit, GUS activity assay can increase promoter. Taken together, these results demonstrate played an important to plants’ its may be affected several genes.